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Gp Crusher How It Works In Poland

DNABinding Dyes The most commonly used DNAbinding dye for realtime PCR is SYBR Green which binds nonspecifically to doublestranded DNA dsDNA SYBR Green exhibits little fluorescence when it is free in solution but its fluorescence increases up to 1000fold when it binds to dsDNA Figure 1 Therefore the overall fluorescence signal from a reaction is proportional to the amount

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